Review



fitc conjugated anti cd54  (Miltenyi Biotec)


Bioz Verified Symbol Miltenyi Biotec is a verified supplier
Bioz Manufacturer Symbol Miltenyi Biotec manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 93
      Buy from Supplier

    Structured Review

    Miltenyi Biotec fitc conjugated anti cd54
    Fitc Conjugated Anti Cd54, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd54+fitc/CD54+(ICAM-1)+Antibody%2C+anti-mouse%2C+FITC%2C+REAfinity/pmc12298679-69-6-13
    Average 93 stars, based on 5 article reviews
    fitc conjugated anti cd54 - by Bioz Stars, 2026-10
    93/100 stars

    Images

    Related Articles

    Staining:

    Article Title: The Plant-Derived Chalcone 2,2′,5′-Trihydroxychalcone Provides Neuroprotection against Toll-Like Receptor 4 Triggered Inflammation in Microglia
    Article Snippet: .. Cells were stained with anti-mouse CD11b-FITC, CD40-FITC, CD54-FITC, and CD68-FITC, or appropriate FITC-conjugated isotype control antibodies (Miltenyi Biotec, UK) as per the manufacturer's instructions and cell surface staining was assessed by flow cytometry (FACSCalibur running CellQuest Pro; Becton Dickinson, UK) and analysed using Flowing software v2.5.1. ..

    Control:

    Article Title: The Plant-Derived Chalcone 2,2′,5′-Trihydroxychalcone Provides Neuroprotection against Toll-Like Receptor 4 Triggered Inflammation in Microglia
    Article Snippet: .. Cells were stained with anti-mouse CD11b-FITC, CD40-FITC, CD54-FITC, and CD68-FITC, or appropriate FITC-conjugated isotype control antibodies (Miltenyi Biotec, UK) as per the manufacturer's instructions and cell surface staining was assessed by flow cytometry (FACSCalibur running CellQuest Pro; Becton Dickinson, UK) and analysed using Flowing software v2.5.1. ..

    Flow Cytometry:

    Article Title: The Plant-Derived Chalcone 2,2′,5′-Trihydroxychalcone Provides Neuroprotection against Toll-Like Receptor 4 Triggered Inflammation in Microglia
    Article Snippet: .. Cells were stained with anti-mouse CD11b-FITC, CD40-FITC, CD54-FITC, and CD68-FITC, or appropriate FITC-conjugated isotype control antibodies (Miltenyi Biotec, UK) as per the manufacturer's instructions and cell surface staining was assessed by flow cytometry (FACSCalibur running CellQuest Pro; Becton Dickinson, UK) and analysed using Flowing software v2.5.1. ..

    Software:

    Article Title: The Plant-Derived Chalcone 2,2′,5′-Trihydroxychalcone Provides Neuroprotection against Toll-Like Receptor 4 Triggered Inflammation in Microglia
    Article Snippet: .. Cells were stained with anti-mouse CD11b-FITC, CD40-FITC, CD54-FITC, and CD68-FITC, or appropriate FITC-conjugated isotype control antibodies (Miltenyi Biotec, UK) as per the manufacturer's instructions and cell surface staining was assessed by flow cytometry (FACSCalibur running CellQuest Pro; Becton Dickinson, UK) and analysed using Flowing software v2.5.1. ..



    Similar Products

    fitc conjugated anti cd54  (Miltenyi Biotec)
    93
    Miltenyi Biotec fitc conjugated anti cd54
    Fitc Conjugated Anti Cd54, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd54+fitc/CD54+(ICAM-1)+Antibody%2C+anti-mouse%2C+FITC%2C+REAfinity/pmc12298679-69-6-13
    Average 93 stars, based on 1 article reviews
    fitc conjugated anti cd54 - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier
    human cd54-fitc antibody  (ImmunoTools)
    90
    ImmunoTools human cd54-fitc antibody
    Human Cd54 Fitc Antibody, supplied by ImmunoTools, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd54+fitc/fitc+labeled+mouse+anti+human+cd54+antibody/pm40325158-306-7-15
    Average 90 stars, based on 1 article reviews
    human cd54-fitc antibody - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    fitc hamster anti-mouse cd54 (clone: 3e2)  (Becton Dickinson)
    90
    Becton Dickinson fitc hamster anti-mouse cd54 (clone: 3e2)
    CDNPs are internalized by 26% of BMN and modulate their expression of CD80 and CD11b. BMC from C57BL6 mice were preincubated with 10 µg/mL CFSE-labeled CDNPs for 45 min and subsequently cultured with or without LPS for 24 hours (A) The percentage of the CFSE-labeled subset among the Ly6G+CD11b+ BMN was determined by flow cytometry. To establish that CFSE-labeled CDNPs were internalized rather than surface-bound, samples were analyzed before and after treatment with trypan blue in initial experiments ( <xref ref-type= Supplementary Figure 3 ). (B) The percentage of CD54 expressing cells in CFSE- (CDNP - ) and CFSE+ (CDNP + ) BMN is shown. (C) Following exposure to CDNPs for 45 min and subsequent culture for 24 h with or without 100ng/mL LPS, BMC were stimulated with 100 ng/mL of CXCL12 for 3 h in transwell plates. The percentage of migrated CDNP - and CDNP + BMN was calculated by flow cytometry. (D) The percentage of CD86 and MHCII expressing cells in CDNP - and CDNP + BMN is shown. Representative histograms for CD80, CD86, MHCII and CD54 are shown in Supplementary Figure 4 . (E, F) The MFI of CD11b (E) and CD80 (F) was assessed in CDNP - and CDNP + BMN. Data are presented as mean ± SD, pooled data from 2 independent experiments with n = 8-12 (2 x 4-6 pseudo-replicates) per group. Significance was determined with a two-way with Bonferroni posttests. *** p < 0.001; **** p < 0.0001. Bars: CDNP - BMN (pale gray), CDNP + BMN (patterned). The incubation periods are illustrated below the graphs. Supplementary Figure 3 provides additional information on the quenching of surface-bound CFSE. The incubation periods are illustrated below the graphs or in (A) on the right side. ns means not significant. " width="250" height="auto" />
    Fitc Hamster Anti Mouse Cd54 (Clone: 3e2), supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd54+fitc/anti+cd54/pmc11794831-63-121-145
    Average 90 stars, based on 1 article reviews
    fitc hamster anti-mouse cd54 (clone: 3e2) - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    cd119 fitc  (Miltenyi Biotec)
    94
    Miltenyi Biotec cd119 fitc
    CDNPs are internalized by 26% of BMN and modulate their expression of CD80 and CD11b. BMC from C57BL6 mice were preincubated with 10 µg/mL CFSE-labeled CDNPs for 45 min and subsequently cultured with or without LPS for 24 hours (A) The percentage of the CFSE-labeled subset among the Ly6G+CD11b+ BMN was determined by flow cytometry. To establish that CFSE-labeled CDNPs were internalized rather than surface-bound, samples were analyzed before and after treatment with trypan blue in initial experiments ( <xref ref-type= Supplementary Figure 3 ). (B) The percentage of CD54 expressing cells in CFSE- (CDNP - ) and CFSE+ (CDNP + ) BMN is shown. (C) Following exposure to CDNPs for 45 min and subsequent culture for 24 h with or without 100ng/mL LPS, BMC were stimulated with 100 ng/mL of CXCL12 for 3 h in transwell plates. The percentage of migrated CDNP - and CDNP + BMN was calculated by flow cytometry. (D) The percentage of CD86 and MHCII expressing cells in CDNP - and CDNP + BMN is shown. Representative histograms for CD80, CD86, MHCII and CD54 are shown in Supplementary Figure 4 . (E, F) The MFI of CD11b (E) and CD80 (F) was assessed in CDNP - and CDNP + BMN. Data are presented as mean ± SD, pooled data from 2 independent experiments with n = 8-12 (2 x 4-6 pseudo-replicates) per group. Significance was determined with a two-way with Bonferroni posttests. *** p < 0.001; **** p < 0.0001. Bars: CDNP - BMN (pale gray), CDNP + BMN (patterned). The incubation periods are illustrated below the graphs. Supplementary Figure 3 provides additional information on the quenching of surface-bound CFSE. The incubation periods are illustrated below the graphs or in (A) on the right side. ns means not significant. " width="250" height="auto" />
    Cd119 Fitc, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd54+fitc/CD54+(ICAM-1)+Antibody%2C+anti-human%2C+REAfinity/pmc11430541-52-4-12
    Average 94 stars, based on 1 article reviews
    cd119 fitc - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier
    fitc-conjugated anti-mouse cd54(i-cam)  (Thermo Fisher)
    90
    Thermo Fisher fitc-conjugated anti-mouse cd54(i-cam)
    CDNPs are internalized by 26% of BMN and modulate their expression of CD80 and CD11b. BMC from C57BL6 mice were preincubated with 10 µg/mL CFSE-labeled CDNPs for 45 min and subsequently cultured with or without LPS for 24 hours (A) The percentage of the CFSE-labeled subset among the Ly6G+CD11b+ BMN was determined by flow cytometry. To establish that CFSE-labeled CDNPs were internalized rather than surface-bound, samples were analyzed before and after treatment with trypan blue in initial experiments ( <xref ref-type= Supplementary Figure 3 ). (B) The percentage of CD54 expressing cells in CFSE- (CDNP - ) and CFSE+ (CDNP + ) BMN is shown. (C) Following exposure to CDNPs for 45 min and subsequent culture for 24 h with or without 100ng/mL LPS, BMC were stimulated with 100 ng/mL of CXCL12 for 3 h in transwell plates. The percentage of migrated CDNP - and CDNP + BMN was calculated by flow cytometry. (D) The percentage of CD86 and MHCII expressing cells in CDNP - and CDNP + BMN is shown. Representative histograms for CD80, CD86, MHCII and CD54 are shown in Supplementary Figure 4 . (E, F) The MFI of CD11b (E) and CD80 (F) was assessed in CDNP - and CDNP + BMN. Data are presented as mean ± SD, pooled data from 2 independent experiments with n = 8-12 (2 x 4-6 pseudo-replicates) per group. Significance was determined with a two-way with Bonferroni posttests. *** p < 0.001; **** p < 0.0001. Bars: CDNP - BMN (pale gray), CDNP + BMN (patterned). The incubation periods are illustrated below the graphs. Supplementary Figure 3 provides additional information on the quenching of surface-bound CFSE. The incubation periods are illustrated below the graphs or in (A) on the right side. ns means not significant. " width="250" height="auto" />
    Fitc Conjugated Anti Mouse Cd54(i Cam), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd54+fitc/pe+conjugated+anti+cd54/pmc11395596-108-14-6
    Average 90 stars, based on 1 article reviews
    fitc-conjugated anti-mouse cd54(i-cam) - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    anti-cd54-fitc (icam-1, yn1/1.7.4  (Thermo Fisher)
    90
    Thermo Fisher anti-cd54-fitc (icam-1, yn1/1.7.4
    CDNPs are internalized by 26% of BMN and modulate their expression of CD80 and CD11b. BMC from C57BL6 mice were preincubated with 10 µg/mL CFSE-labeled CDNPs for 45 min and subsequently cultured with or without LPS for 24 hours (A) The percentage of the CFSE-labeled subset among the Ly6G+CD11b+ BMN was determined by flow cytometry. To establish that CFSE-labeled CDNPs were internalized rather than surface-bound, samples were analyzed before and after treatment with trypan blue in initial experiments ( <xref ref-type= Supplementary Figure 3 ). (B) The percentage of CD54 expressing cells in CFSE- (CDNP - ) and CFSE+ (CDNP + ) BMN is shown. (C) Following exposure to CDNPs for 45 min and subsequent culture for 24 h with or without 100ng/mL LPS, BMC were stimulated with 100 ng/mL of CXCL12 for 3 h in transwell plates. The percentage of migrated CDNP - and CDNP + BMN was calculated by flow cytometry. (D) The percentage of CD86 and MHCII expressing cells in CDNP - and CDNP + BMN is shown. Representative histograms for CD80, CD86, MHCII and CD54 are shown in Supplementary Figure 4 . (E, F) The MFI of CD11b (E) and CD80 (F) was assessed in CDNP - and CDNP + BMN. Data are presented as mean ± SD, pooled data from 2 independent experiments with n = 8-12 (2 x 4-6 pseudo-replicates) per group. Significance was determined with a two-way with Bonferroni posttests. *** p < 0.001; **** p < 0.0001. Bars: CDNP - BMN (pale gray), CDNP + BMN (patterned). The incubation periods are illustrated below the graphs. Supplementary Figure 3 provides additional information on the quenching of surface-bound CFSE. The incubation periods are illustrated below the graphs or in (A) on the right side. ns means not significant. " width="250" height="auto" />
    Anti Cd54 Fitc (Icam 1, Yn1/1.7.4, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd54+fitc/pm39201584-339-61-64
    Average 90 stars, based on 1 article reviews
    anti-cd54-fitc (icam-1, yn1/1.7.4 - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    fitc-labeled anti-cd54 antibody  (Agilent technologies)
    90
    Agilent technologies fitc-labeled anti-cd54 antibody
    CDNPs are internalized by 26% of BMN and modulate their expression of CD80 and CD11b. BMC from C57BL6 mice were preincubated with 10 µg/mL CFSE-labeled CDNPs for 45 min and subsequently cultured with or without LPS for 24 hours (A) The percentage of the CFSE-labeled subset among the Ly6G+CD11b+ BMN was determined by flow cytometry. To establish that CFSE-labeled CDNPs were internalized rather than surface-bound, samples were analyzed before and after treatment with trypan blue in initial experiments ( <xref ref-type= Supplementary Figure 3 ). (B) The percentage of CD54 expressing cells in CFSE- (CDNP - ) and CFSE+ (CDNP + ) BMN is shown. (C) Following exposure to CDNPs for 45 min and subsequent culture for 24 h with or without 100ng/mL LPS, BMC were stimulated with 100 ng/mL of CXCL12 for 3 h in transwell plates. The percentage of migrated CDNP - and CDNP + BMN was calculated by flow cytometry. (D) The percentage of CD86 and MHCII expressing cells in CDNP - and CDNP + BMN is shown. Representative histograms for CD80, CD86, MHCII and CD54 are shown in Supplementary Figure 4 . (E, F) The MFI of CD11b (E) and CD80 (F) was assessed in CDNP - and CDNP + BMN. Data are presented as mean ± SD, pooled data from 2 independent experiments with n = 8-12 (2 x 4-6 pseudo-replicates) per group. Significance was determined with a two-way with Bonferroni posttests. *** p < 0.001; **** p < 0.0001. Bars: CDNP - BMN (pale gray), CDNP + BMN (patterned). The incubation periods are illustrated below the graphs. Supplementary Figure 3 provides additional information on the quenching of surface-bound CFSE. The incubation periods are illustrated below the graphs or in (A) on the right side. ns means not significant. " width="250" height="auto" />
    Fitc Labeled Anti Cd54 Antibody, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd54+fitc/pmc11247007-86-17-20
    Average 90 stars, based on 1 article reviews
    fitc-labeled anti-cd54 antibody - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    fitc anti mouse cd54 antibody  (Elabscience Biotechnology)
    92
    Elabscience Biotechnology fitc anti mouse cd54 antibody
    CDNPs are internalized by 26% of BMN and modulate their expression of CD80 and CD11b. BMC from C57BL6 mice were preincubated with 10 µg/mL CFSE-labeled CDNPs for 45 min and subsequently cultured with or without LPS for 24 hours (A) The percentage of the CFSE-labeled subset among the Ly6G+CD11b+ BMN was determined by flow cytometry. To establish that CFSE-labeled CDNPs were internalized rather than surface-bound, samples were analyzed before and after treatment with trypan blue in initial experiments ( <xref ref-type= Supplementary Figure 3 ). (B) The percentage of CD54 expressing cells in CFSE- (CDNP - ) and CFSE+ (CDNP + ) BMN is shown. (C) Following exposure to CDNPs for 45 min and subsequent culture for 24 h with or without 100ng/mL LPS, BMC were stimulated with 100 ng/mL of CXCL12 for 3 h in transwell plates. The percentage of migrated CDNP - and CDNP + BMN was calculated by flow cytometry. (D) The percentage of CD86 and MHCII expressing cells in CDNP - and CDNP + BMN is shown. Representative histograms for CD80, CD86, MHCII and CD54 are shown in Supplementary Figure 4 . (E, F) The MFI of CD11b (E) and CD80 (F) was assessed in CDNP - and CDNP + BMN. Data are presented as mean ± SD, pooled data from 2 independent experiments with n = 8-12 (2 x 4-6 pseudo-replicates) per group. Significance was determined with a two-way with Bonferroni posttests. *** p < 0.001; **** p < 0.0001. Bars: CDNP - BMN (pale gray), CDNP + BMN (patterned). The incubation periods are illustrated below the graphs. Supplementary Figure 3 provides additional information on the quenching of surface-bound CFSE. The incubation periods are illustrated below the graphs or in (A) on the right side. ns means not significant. " width="250" height="auto" />
    Fitc Anti Mouse Cd54 Antibody, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd54+fitc/FITC+Anti-Mouse+CD54+Antibody/pm38810101-253-58-62
    Average 92 stars, based on 1 article reviews
    fitc anti mouse cd54 antibody - by Bioz Stars, 2026-10
    92/100 stars
    		  Buy from Supplier
    anti mouse cd54 antibody  (Elabscience Biotechnology)
    92
    Elabscience Biotechnology anti mouse cd54 antibody
    CDNPs are internalized by 26% of BMN and modulate their expression of CD80 and CD11b. BMC from C57BL6 mice were preincubated with 10 µg/mL CFSE-labeled CDNPs for 45 min and subsequently cultured with or without LPS for 24 hours (A) The percentage of the CFSE-labeled subset among the Ly6G+CD11b+ BMN was determined by flow cytometry. To establish that CFSE-labeled CDNPs were internalized rather than surface-bound, samples were analyzed before and after treatment with trypan blue in initial experiments ( <xref ref-type= Supplementary Figure 3 ). (B) The percentage of CD54 expressing cells in CFSE- (CDNP - ) and CFSE+ (CDNP + ) BMN is shown. (C) Following exposure to CDNPs for 45 min and subsequent culture for 24 h with or without 100ng/mL LPS, BMC were stimulated with 100 ng/mL of CXCL12 for 3 h in transwell plates. The percentage of migrated CDNP - and CDNP + BMN was calculated by flow cytometry. (D) The percentage of CD86 and MHCII expressing cells in CDNP - and CDNP + BMN is shown. Representative histograms for CD80, CD86, MHCII and CD54 are shown in Supplementary Figure 4 . (E, F) The MFI of CD11b (E) and CD80 (F) was assessed in CDNP - and CDNP + BMN. Data are presented as mean ± SD, pooled data from 2 independent experiments with n = 8-12 (2 x 4-6 pseudo-replicates) per group. Significance was determined with a two-way with Bonferroni posttests. *** p < 0.001; **** p < 0.0001. Bars: CDNP - BMN (pale gray), CDNP + BMN (patterned). The incubation periods are illustrated below the graphs. Supplementary Figure 3 provides additional information on the quenching of surface-bound CFSE. The incubation periods are illustrated below the graphs or in (A) on the right side. ns means not significant. " width="250" height="auto" />
    Anti Mouse Cd54 Antibody, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd54+fitc/FITC+Anti-Mouse+CD54+Antibody/pmc11304236-181-56-59
    Average 92 stars, based on 1 article reviews
    anti mouse cd54 antibody - by Bioz Stars, 2026-10
    92/100 stars
    		  Buy from Supplier
    fitc anti‑mouse cd54 antibody  (Elabscience Biotechnology)
    90
    Elabscience Biotechnology fitc anti‑mouse cd54 antibody
    Organ damage and immunosuppression in LPS‐induced septic mice. A–D) ALT, AST, BUN in serum of mice, MPO activity in lung tissue. E–G) H&E for histopathological evaluation of Liver, Kidney, and Spleen. H,L) Splenocyte apoptosis was detected by flow cytometry using <t>FITC‐Annexin</t> V and PI staining, I,M) Flow cytometric detection <t>of</t> <t>CD3</t> + CD4 + T cells in spleens. J,N) Flow cytometric detection of CD3 + CD8 + T cells in spleens. K,O) Flow cytometric detection of CD3 − CD19 + B cells in spleens. Scale bars, 100 µm. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001; ns, not significant. Error bars indicate SD.
    Fitc Anti‑Mouse Cd54 Antibody, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd54+fitc/fitc+anti+mouse+cd3+antibody/pmc11304236-181-55-59
    Average 90 stars, based on 1 article reviews
    fitc anti‑mouse cd54 antibody - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    CDNPs are internalized by 26% of BMN and modulate their expression of CD80 and CD11b. BMC from C57BL6 mice were preincubated with 10 µg/mL CFSE-labeled CDNPs for 45 min and subsequently cultured with or without LPS for 24 hours (A) The percentage of the CFSE-labeled subset among the Ly6G+CD11b+ BMN was determined by flow cytometry. To establish that CFSE-labeled CDNPs were internalized rather than surface-bound, samples were analyzed before and after treatment with trypan blue in initial experiments ( <xref ref-type= Supplementary Figure 3 ). (B) The percentage of CD54 expressing cells in CFSE- (CDNP - ) and CFSE+ (CDNP + ) BMN is shown. (C) Following exposure to CDNPs for 45 min and subsequent culture for 24 h with or without 100ng/mL LPS, BMC were stimulated with 100 ng/mL of CXCL12 for 3 h in transwell plates. The percentage of migrated CDNP - and CDNP + BMN was calculated by flow cytometry. (D) The percentage of CD86 and MHCII expressing cells in CDNP - and CDNP + BMN is shown. Representative histograms for CD80, CD86, MHCII and CD54 are shown in Supplementary Figure 4 . (E, F) The MFI of CD11b (E) and CD80 (F) was assessed in CDNP - and CDNP + BMN. Data are presented as mean ± SD, pooled data from 2 independent experiments with n = 8-12 (2 x 4-6 pseudo-replicates) per group. Significance was determined with a two-way with Bonferroni posttests. *** p < 0.001; **** p < 0.0001. Bars: CDNP - BMN (pale gray), CDNP + BMN (patterned). The incubation periods are illustrated below the graphs. Supplementary Figure 3 provides additional information on the quenching of surface-bound CFSE. The incubation periods are illustrated below the graphs or in (A) on the right side. ns means not significant. " width="100%" height="100%">

    Journal: Frontiers in Immunology

    Article Title: Nanoparticles containing intracellular proteins modulate neutrophil functional and phenotypic heterogeneity

    doi: 10.3389/fimmu.2024.1494400

    Figure Lengend Snippet: CDNPs are internalized by 26% of BMN and modulate their expression of CD80 and CD11b. BMC from C57BL6 mice were preincubated with 10 µg/mL CFSE-labeled CDNPs for 45 min and subsequently cultured with or without LPS for 24 hours (A) The percentage of the CFSE-labeled subset among the Ly6G+CD11b+ BMN was determined by flow cytometry. To establish that CFSE-labeled CDNPs were internalized rather than surface-bound, samples were analyzed before and after treatment with trypan blue in initial experiments ( Supplementary Figure 3 ). (B) The percentage of CD54 expressing cells in CFSE- (CDNP - ) and CFSE+ (CDNP + ) BMN is shown. (C) Following exposure to CDNPs for 45 min and subsequent culture for 24 h with or without 100ng/mL LPS, BMC were stimulated with 100 ng/mL of CXCL12 for 3 h in transwell plates. The percentage of migrated CDNP - and CDNP + BMN was calculated by flow cytometry. (D) The percentage of CD86 and MHCII expressing cells in CDNP - and CDNP + BMN is shown. Representative histograms for CD80, CD86, MHCII and CD54 are shown in Supplementary Figure 4 . (E, F) The MFI of CD11b (E) and CD80 (F) was assessed in CDNP - and CDNP + BMN. Data are presented as mean ± SD, pooled data from 2 independent experiments with n = 8-12 (2 x 4-6 pseudo-replicates) per group. Significance was determined with a two-way with Bonferroni posttests. *** p < 0.001; **** p < 0.0001. Bars: CDNP - BMN (pale gray), CDNP + BMN (patterned). The incubation periods are illustrated below the graphs. Supplementary Figure 3 provides additional information on the quenching of surface-bound CFSE. The incubation periods are illustrated below the graphs or in (A) on the right side. ns means not significant.

    Article Snippet: The following fluorescent-labeled antibodies were used: Anti-Histone H3 (citrulline R2 + R8 + R17) Primary Antibody (Polyclonal), FITC Anti-Myeloperoxidase antibody (clone: 2D4) (both from Abcam, UK); APC anti-mouse CD86 Antibody (clone: GL-1), APC-Cy7 anti-mouse CD86 Antibody (clone: GL-1), Brilliant Violet 605TM antimouse/human CD11b Antibody (clone: M1/70), Pacific BlueTM anti-mouse I-Ab Antibody (MHC II) (clone: AF6-120.1), Pacific BlueTM anti-mouse Ly-6G Antibody (clone: 1A8), PE anti-mouse CD80 Antibody (clone: 16-10A1), PerCP/Cy5.5 anti-mouse Ly-6G Antibody (clone: 1A8), APC anti-mouse Ly-6G Antibody (clone: 1A8), Brilliant Violet 421TM antimouse/human CD11b Antibody (clone: M1/70) (all from BioLegend, USA); Anti-mouse CD16/32 Antibody (clone: 93) (from BioLegend, USA or Thermo Fisher Scientific, USA); APC Hamster Anti-Mouse CD54 (clone: 3E2), APC Rat Anti-CD11b (clone: M1/70), APC-Cy7 Rat Anti-CD11b (clone: M1/70), FITC Hamster Anti-Mouse CD54 (clone: 3E2), Fixable Viability Stain 570, PE Hamster Anti-Mouse CD54 (clone: 3E2), PE Rat Anti-Mouse Ly-6G (clone: 1A8) (all from Becton Dickinson, USA), PE CD11b Monoclonal Antibody (clone: M1/70), Fixable Viability Dye eFluor 780; Alexa Fluor 700 Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody [all from Thermo Fisher Scientific (USA)].

    Techniques: Expressing, Labeling, Cell Culture, Flow Cytometry, Incubation

    20% of BMN mature to a CD86+/MHCII+/CD54+ subset during culture. (A–D) BMC from C57BL6 mice were incubated with 10 µg/mL CDNPs for 45 min, 3 hours or 24 hours. (A) The MFI of CD80 expression and (B–D) The percentage of MHCII, CD86 and CD54 expressing BMN was determined by flow cytometric analysis. Representative histograms for CD80, CD86, MHCII, and CD54 are shown in <xref ref-type= Supplementary Figure 2 . (E) Following exposure to CDNPs for 45 min and subsequent culture for 24 hours with or without 100 ng/mL LPS, BMC were stimulated with 100 ng/mL of CXCL12 for 3 h in transwell plates. The percentage of migrated BMN was calculated by flow cytometry. Data are expressed as mean ± SD, pooled data from 2 independent experiments with n = 8-12 (2 x 4-6 pseudo-replicates) per group. Significance was determined with a two-way ANOVA with Bonferroni post-tests. * p < 0.05, **** p < 0.0001, adjusted significance level α (k=2) = 0.025. Bars: PBS (pale gray), CDNP (dark gray). The incubation periods are illustrated below the graphs. ns means not significant. " width="100%" height="100%">

    Journal: Frontiers in Immunology

    Article Title: Nanoparticles containing intracellular proteins modulate neutrophil functional and phenotypic heterogeneity

    doi: 10.3389/fimmu.2024.1494400

    Figure Lengend Snippet: 20% of BMN mature to a CD86+/MHCII+/CD54+ subset during culture. (A–D) BMC from C57BL6 mice were incubated with 10 µg/mL CDNPs for 45 min, 3 hours or 24 hours. (A) The MFI of CD80 expression and (B–D) The percentage of MHCII, CD86 and CD54 expressing BMN was determined by flow cytometric analysis. Representative histograms for CD80, CD86, MHCII, and CD54 are shown in Supplementary Figure 2 . (E) Following exposure to CDNPs for 45 min and subsequent culture for 24 hours with or without 100 ng/mL LPS, BMC were stimulated with 100 ng/mL of CXCL12 for 3 h in transwell plates. The percentage of migrated BMN was calculated by flow cytometry. Data are expressed as mean ± SD, pooled data from 2 independent experiments with n = 8-12 (2 x 4-6 pseudo-replicates) per group. Significance was determined with a two-way ANOVA with Bonferroni post-tests. * p < 0.05, **** p < 0.0001, adjusted significance level α (k=2) = 0.025. Bars: PBS (pale gray), CDNP (dark gray). The incubation periods are illustrated below the graphs. ns means not significant.

    Article Snippet: The following fluorescent-labeled antibodies were used: Anti-Histone H3 (citrulline R2 + R8 + R17) Primary Antibody (Polyclonal), FITC Anti-Myeloperoxidase antibody (clone: 2D4) (both from Abcam, UK); APC anti-mouse CD86 Antibody (clone: GL-1), APC-Cy7 anti-mouse CD86 Antibody (clone: GL-1), Brilliant Violet 605TM antimouse/human CD11b Antibody (clone: M1/70), Pacific BlueTM anti-mouse I-Ab Antibody (MHC II) (clone: AF6-120.1), Pacific BlueTM anti-mouse Ly-6G Antibody (clone: 1A8), PE anti-mouse CD80 Antibody (clone: 16-10A1), PerCP/Cy5.5 anti-mouse Ly-6G Antibody (clone: 1A8), APC anti-mouse Ly-6G Antibody (clone: 1A8), Brilliant Violet 421TM antimouse/human CD11b Antibody (clone: M1/70) (all from BioLegend, USA); Anti-mouse CD16/32 Antibody (clone: 93) (from BioLegend, USA or Thermo Fisher Scientific, USA); APC Hamster Anti-Mouse CD54 (clone: 3E2), APC Rat Anti-CD11b (clone: M1/70), APC-Cy7 Rat Anti-CD11b (clone: M1/70), FITC Hamster Anti-Mouse CD54 (clone: 3E2), Fixable Viability Stain 570, PE Hamster Anti-Mouse CD54 (clone: 3E2), PE Rat Anti-Mouse Ly-6G (clone: 1A8) (all from Becton Dickinson, USA), PE CD11b Monoclonal Antibody (clone: M1/70), Fixable Viability Dye eFluor 780; Alexa Fluor 700 Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody [all from Thermo Fisher Scientific (USA)].

    Techniques: Incubation, Expressing, Flow Cytometry

    Organ damage and immunosuppression in LPS‐induced septic mice. A–D) ALT, AST, BUN in serum of mice, MPO activity in lung tissue. E–G) H&E for histopathological evaluation of Liver, Kidney, and Spleen. H,L) Splenocyte apoptosis was detected by flow cytometry using FITC‐Annexin V and PI staining, I,M) Flow cytometric detection of CD3 + CD4 + T cells in spleens. J,N) Flow cytometric detection of CD3 + CD8 + T cells in spleens. K,O) Flow cytometric detection of CD3 − CD19 + B cells in spleens. Scale bars, 100 µm. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001; ns, not significant. Error bars indicate SD.

    Journal: Advanced Science

    Article Title: A Novel Drug Candidate for Sepsis Targeting Heparanase by Inhibiting Cytokine Storm

    doi: 10.1002/advs.202403337

    Figure Lengend Snippet: Organ damage and immunosuppression in LPS‐induced septic mice. A–D) ALT, AST, BUN in serum of mice, MPO activity in lung tissue. E–G) H&E for histopathological evaluation of Liver, Kidney, and Spleen. H,L) Splenocyte apoptosis was detected by flow cytometry using FITC‐Annexin V and PI staining, I,M) Flow cytometric detection of CD3 + CD4 + T cells in spleens. J,N) Flow cytometric detection of CD3 + CD8 + T cells in spleens. K,O) Flow cytometric detection of CD3 − CD19 + B cells in spleens. Scale bars, 100 µm. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001; ns, not significant. Error bars indicate SD.

    Article Snippet: Rb a Heparanase 1 (Bioss, bs‐1541R); GAPDH Monoclonal Antibody (Immuno Way, YM3029); Goat Anti‐Rabbit IgG H&L (Alexa Fluor 594) (abcam, ab150080); Goat Anti‐Rabbit IgG (Elabscience, E‐AB‐1034); Goat Anti‐Mouse IgG (Elabscience, E‐AB‐1035); APC anti mouse CD3 (Biolegend, 100235); FITC anti mouse CD4 (Biolegend, 100509); PE anti mouse CD8a (Biolegend, 100707); PE anti mouse CD19 (Biolegend, 115507); FITC Anti‐mouse CD54 antibody (Elabscience, E‐AB‐F1018C); FITC Anti‐Human CD54 antibody (Elabscience, E‐AB‐F1043C); FITC Anti‐mouse CD106 antibody (Elabscience, E‐AB‐F1091M); FITC‐WGA Lectin (GeneTex, GTX01502); Annexin V‐FITC/PI Apoptosis Kit (Elabscience, E‐CK‐A211); Aspartate aminotransferase Assay Kit (Nanjing Jiancheng Bioengineering Institute, C010‐2‐1); Alanine aminotransferase Assay Kit (Nanjing Jiancheng Bioengineering Institute, C009‐2‐1); Urea Assay Kit (Nanjing Jiancheng Bioengineering Institute, C013‐2‐1); Myeloperoxidase Activity Assay Kit (Elabscience, E‐BC‐K074‐M); Chemiluminescent HRP Substrate (Millipore, WBKLS0100); DAPI (solarbio, C0065); RIPA Tissue/cell lysate solution (solarbio, R0020); Cell Counting Kit‐8 (Dojindo, CK04); Immunohistochemistry staining kit (Bioss, SP‐0023); 0.25% Trypsin‐EDTA (Gibco, 25200‐072); Fetal Bovine Serum (Procell, 14210–500); DMEM High‐glucose medium (Solarbio, 12100); TNF‐α, Human (GenScript, Z01001‐10); LPS (Escherichia coli O55:B5) (Sigma, L2880); Suramin (GlpBio, GC16832); TNF‐α ELISA MAXTM Deluxe Set (BioLegend, 430904); IL‐6 ELISA MAXTM Deluxe Set (BioLegend, 431304); IL‐1β ELISA MAXTM Deluxe Set (BioLegend, 432604); FITC anti mouse F4/80 (Biolegend, 123107); APC anti mouse CD86 (Biolegend, 105113).

    Techniques: Activity Assay, Flow Cytometry, Staining